Bio-Connect
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Rat heart tissue, Rat liver tissue All lanes: ATP5B antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Rat heart tissue, Rat liver tissue All lanes: ATP5B antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Rat heart tissue, Rat liver tissue All lanes: ATP5B antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa

ATP5B Antibody

CSB-PA002350EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATP5F1B
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Cusabio
  • Product Name
    ATP5B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID506
  • Target name
    ATP5F1B
  • Target description
    ATP synthase F1 subunit beta
  • Target synonyms
    ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATP5B; ATPMB; ATPSB; epididymis secretory protein Li 271; HEL-S-271; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetATP5F1B
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetATP5F1B
  • SizePrice
Product group Antibodies
Assay Genie
TargetATP5F1B
  • SizePrice
Product group Antibodies
MedChem Express
TargetATP5F1B
  • SizePrice
Immunohistochemical staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody HPA001520 (A) shows similar protein distribution across tissues to independent antibody HPA001528 (B).
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetATP5F1B
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetATP5F1B
  • SizePrice
Figure 1. Western blot analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1B antigen affinity purified polyclonal antibody (Catalog # A32270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1B at approximately 54 kDa. The expected band size for ATP5F1B is at 57 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetATP5F1B
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetATP5F1B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetATP5F1B
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-ATP5BY058293
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice