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ProductsBack/Goat anti-ATP5B (aa15162)

Goat anti-ATP5B (aa15162)

EB12459
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATP5F1B
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATP5B (aa15162) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID506
  • Target name
    ATP5F1B
  • Target description
    ATP synthase F1 subunit beta
  • Target synonyms
    ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATP5B; ATPMB; ATPSB; epididymis secretory protein Li 271; HEL-S-271; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001677.2. GeneIDs all Nonhuman: 11947 (mouse); 171374 (rat);. Purification: Antigen affinity purified. Names and symbols: ATP5B; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATPMB; ATPSB; ATP synthase subunit beta, mitochondrial; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1B antigen affinity purified polyclonal antibody (Catalog # A32270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1B at approximately 54 kDa. The expected band size for ATP5F1B is at 57 kDa.
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