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ProductsBack/ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1B10]
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500837, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500837, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500837, Dilution 1:50)

ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CF500837
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetATP5F1B
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100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1B10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B10
  • Gene ID506
  • Target name
    ATP5F1B
  • Target description
    ATP synthase F1 subunit beta
  • Target synonyms
    ATP5B, ATPMB, ATPSB, HEL-S-271, HUMOP2, ATP synthase F(1) complex subunit beta, mitochondrial, ATP synthase subunit beta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit, mitochondrial ATP synthetase, beta subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ATP5B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
  • Reactivity
    Canine, Human, Monkey, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Rat heart tissue, Rat liver tissue All lanes: ATP5B antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
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Figure 1. Western blot analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1B antigen affinity purified polyclonal antibody (Catalog # A32270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1B at approximately 54 kDa. The expected band size for ATP5F1B is at 57 kDa.
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