More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI9E3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B. Positive lysates LY400637 (100ug) and LC400637 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B. Positive lysates LY400637 (100ug) and LC400637 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B. Positive lysates LY400637 (100ug) and LC400637 (20ug) can be purchased separately from OriGene.

ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI9E3]

CF500835
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetATP5F1B
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI9E3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI9E3
  • Gene ID506
  • Target name
    ATP5F1B
  • Target description
    ATP synthase F1 subunit beta
  • Target synonyms
    ATP5B, ATPMB, ATPSB, HEL-S-271, HUMOP2, ATP synthase F(1) complex subunit beta, mitochondrial, ATP synthase subunit beta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit, mitochondrial ATP synthetase, beta subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ATP5B mouse monoclonal antibody, clone OTI9E3 (formerly 9E3)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Rat heart tissue, Rat liver tissue All lanes: ATP5B antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
Product group Antibodies
Cusabio
ATP5B AntibodyCSB-PA002350EA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetATP5F1B
  • SizePrice
Product group Antibodies
Assay Genie
ATPB AntibodyCAB5769
ReactivityHuman
TargetATP5F1B
  • SizePrice
Figure 1. Western blot analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1B antigen affinity purified polyclonal antibody (Catalog # A32270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1B at approximately 54 kDa. The expected band size for ATP5F1B is at 57 kDa.
Product group Antibodies
Boster Bio
Anti-ATP5F1B Antibody Picoband(r)A32270-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetATP5F1B
  • SizePrice
Anti-ATP5B Antibody
Product group Antibodies
Antibodies.com
Anti-ATP5B AntibodyA31039
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
ATPB Antibody (YA2214, Rabbit)HY-P82469
TargetATP5F1B
  • SizePrice
Immunohistochemical staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody HPA001520 (A) shows similar protein distribution across tissues to independent antibody HPA001528 (B).
Product group Antibodies
Atlas Antibodies
Anti-ATP5B AntibodyHPA001520
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetATP5F1B
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-ATP5B (aa15162)EB12459
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetATP5F1B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ATP5B / ATP Synthase Beta AntibodyLS-C482498
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetATP5F1B
  • SizePrice
Product group Antibodies
Biomatik
ATP5B Polyclonal AntibodyCAC14816
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetATP5F1B
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-ATP5BY058293
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice