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ATP6V1B1 Antibody

LS-C348977
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetATP6V1B1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATP6V1B1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 56kDa, while the observed MW by Western blot was 57kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 56kDa, while the observed MW by Western blot was 57kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID525
  • Target name
    ATP6V1B1
  • Target description
    ATPase H+ transporting V1 subunit B1
  • Target synonyms
    ATP6B1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; DRTA2; endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; H+-ATPase beta 1 subunit; RTA1B; vacuolar proton pump 3; vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3; VATB; V-ATPase B1 subunit; V-ATPase subunit B 1; VMA2; VPP3; V-type proton ATPase subunit B, kidney isoform
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP6V1B1 using anti-ATP6V1B1 antibody (A06073-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1B1 antigen affinity purified polyclonal antibody (Catalog # A06073-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP6V1B1 at approximately 69 kDa. The expected band size for ATP6V1B1 is at 57 kDa.
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