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ATR antibody

GTX10327
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetATR
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.033 - 0.067 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID545
  • Target name
    ATR
  • Target description
    ATR checkpoint kinase
  • Target synonyms
    FCTCS, FRP1, MEC1, SCKL, SCKL1, serine/threonine-protein kinase ATR, ATR serine/threonine kinase, FRAP-related protein-1, MEC1, mitosis entry checkpoint 1, homolog, ataxia telangiectasia and Rad3-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13535
  • Protein Name
    Serine/threonine-protein kinase ATR
  • Scientific Description
    The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Rankin AM, Sarkar S, Faller DV. Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells. J Cell Physiol. 2012,227(6):2586-94. doi: 10.1002/jcp.22997
    Read this paper

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Figure 1. IF analysis of ATR using anti-ATR antibody (A00262-2). ATR was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 microg/mL rabbit anti-ATR Antibody (A00262-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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