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AURKB antibody

10R-1182
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    AURKB antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Aurora B AURKB Rabbit Monoclonal AntibodyM00762-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetAURKB
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Formalin-fixed and paraffin embedded human ovary labeled with Anti-Aurora B/STK-1 Polyclonal Antibody, Unconjugated (bs-2445R) at 1:200 followed by conjugation to the secondary antibody
Product group Antibodies
References
Bioss Antibodies
Aurora B Polyclonal AntibodyBS-2445R
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TargetAURKB
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Biomatik
Aurkb Polyclonal AntibodyCAC07483
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TargetAURKB
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Assay Genie
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LifeSpan BioSciences
AURKB / Aurora-B Antibody (N-Terminus)LS-C358921
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Antibodies
Cusabio
AURKB AntibodyCSB-PA000926
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAURKB
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Product group Antibodies
MedChem Express
Aurora B Antibody (YA594, Rabbit)HY-P80562
TargetAURKB
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.
Product group Antibodies
Atlas Antibodies
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Product group Antibodies
Everest Biotech
Goat anti-Aurora Kinase BEB07260
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Product group Antibodies
References
GeneTex
Aurora B antibodyGTX130211
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TargetAURKB
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