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ProductsBack/AURKB Antibody
Western Blot analysis of COS7 cells using ARK-2 Polyclonal Antibody
Western Blot analysis of COS7 cells using ARK-2 Polyclonal Antibody
Western Blot analysis of COS7 cells using ARK-2 Polyclonal Antibody

AURKB Antibody

CSB-PA000926
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetAURKB
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Overview

  • Supplier
    Cusabio
  • Product Name
    AURKB Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9212
  • Target name
    AURKB
  • Target description
    aurora kinase B
  • Target synonyms
    AIK2; AIM1; AIM-1; ARK2; ARK-2; AurB; aurkb-sv1; aurkb-sv2; aurora- and Ipl1-like midbody-associated protein 1; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora/IPL1-related kinase 2; aurora-1; aurora-B; aurora-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine-protein kinase 12; serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; STK-1; STK12; STK5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GD4
  • Protein Name
    Aurora kinase B
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Aurora B AURKB Rabbit Monoclonal AntibodyM00762-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetAURKB
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Formalin-fixed and paraffin embedded human ovary labeled with Anti-Aurora B/STK-1 Polyclonal Antibody, Unconjugated (bs-2445R) at 1:200 followed by conjugation to the secondary antibody
Product group Antibodies
References
Bioss Antibodies
Aurora B Polyclonal AntibodyBS-2445R
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetAURKB
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Product group Antibodies
Biomatik
Aurkb Polyclonal AntibodyCAC07483
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetAURKB
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Assay Genie
RBBP8 AntibodyCAB10201
TargetAURKB
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Product group Antibodies
LifeSpan BioSciences
AURKB / Aurora-B Antibody (N-Terminus)LS-C358921
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetAURKB
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Product group Antibodies
Biosynth
AURKB antibody10R-1182
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Product group Antibodies
MedChem Express
Aurora B Antibody (YA594, Rabbit)HY-P80562
TargetAURKB
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.
Product group Antibodies
Atlas Antibodies
Anti-AURKB AntibodyHPA037708
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetAURKB
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Product group Antibodies
Everest Biotech
Goat anti-Aurora Kinase BEB07260
ApplicationsWestern Blot, ELISA
TargetAURKB
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Product group Antibodies
References
GeneTex
Aurora B antibodyGTX130211
ApplicationsWestern Blot
TargetAURKB
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