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ProductsBack/AURKB / Aurora-B Antibody (aa283-313, PE)

AURKB / Aurora-B Antibody (aa283-313, PE)

LS-C252107
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAURKB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AURKB / Aurora-B Antibody (aa283-313, PE)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID9212
  • Target name
    AURKB
  • Target description
    aurora kinase B
  • Target synonyms
    AIK2; AIM1; AIM-1; ARK2; ARK-2; AurB; aurkb-sv1; aurkb-sv2; aurora- and Ipl1-like midbody-associated protein 1; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora/IPL1-related kinase 2; aurora-1; aurora-B; aurora-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine-protein kinase 12; serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; STK-1; STK12; STK5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
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