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ProductsBack/Aurora B antibody [13E8D3]
WB analysis of truncated AURKB recombinant protein (1) and SKN-SH cell lysate(2) using GTX83084 Aurora B antibody [13E8D3].
WB analysis of truncated AURKB recombinant protein (1) and SKN-SH cell lysate(2) using GTX83084 Aurora B antibody [13E8D3].
WB analysis of truncated AURKB recombinant protein (1) and SKN-SH cell lysate(2) using GTX83084 Aurora B antibody [13E8D3].

Aurora B antibody [13E8D3]

GTX83084
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetAURKB
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aurora B antibody [13E8D3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    13E8D3
  • Conjugate
    Unconjugated
  • Gene ID9212
  • Target name
    AURKB
  • Target description
    aurora kinase B
  • Target synonyms
    AIK2; AIM1; AIM-1; ARK2; ARK-2; AurB; aurkb-sv1; aurkb-sv2; aurora- and Ipl1-like midbody-associated protein 1; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora/IPL1-related kinase 2; aurora-1; aurora-B; aurora-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine-protein kinase 12; serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; STK-1; STK12; STK5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96GD4
  • Protein Name
    Aurora kinase B
  • Scientific Description
    This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
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Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen
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