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ProductsBack/B-Cell Activation Factor Receptor (BAFFR) Polyclonal Antibody

B-Cell Activation Factor Receptor (BAFFR) Polyclonal Antibody

CAU28196
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNFRSF13C
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Overview

  • Supplier
    Biomatik
  • Product Name
    B-Cell Activation Factor Receptor (BAFFR) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID115650
  • Target name
    TNFRSF13C
  • Target description
    TNF receptor superfamily member 13C
  • Target synonyms
    B cell-activating factor receptor; BAFF receptor; BAFFR; BAFF-R; BLyS receptor 3; BROMIX; CD268; CVID4; prolixin; tumor necrosis factor receptor superfamily member 13C
  • Host
    Rabbit
  • Protein IDQ96RJ3
  • Protein Name
    Tumor necrosis factor receptor superfamily member 13C
  • Scientific Description
    The B-Cell Activation Factor Receptor (BAFFR) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, ICC, IHC-P, IHC-F, ELISA.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19KD. The expected band size for BAFF Receptor is at 19KD.
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