
Figure 1. Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19KD. The expected band size for BAFF Receptor is at 19KD.
Anti-BAFF Receptor/TNFRSF13C Antibody Picoband(r)
A02865-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTNFRSF13C
Overview
- SupplierBoster Bio
- Product NameAnti-BAFF Receptor/TNFRSF13C Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID115650
- Target nameTNFRSF13C
- Target descriptionTNF receptor superfamily member 13C
- Target synonymsB cell-activating factor receptor; BAFF receptor; BAFFR; BAFF-R; BLyS receptor 3; BROMIX; CD268; CVID4; prolixin; tumor necrosis factor receptor superfamily member 13C
- HostRabbit
- IsotypeIgG
- Protein IDQ96RJ3
- Protein NameTumor necrosis factor receptor superfamily member 13C
- Scientific DescriptionBoster Bio Anti-BAFF Receptor/TNFRSF13C Antibody Picoband® catalog # A02865-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203