ICC/IF analysis of MCF-7 (left) and HepG2 (right) cells using GTX83134 B-Raf antibody [1H12]. Green : B-Raf Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
![IHC-P analysis of human testis tissue using GTX83134 B-Raf antibody [1H12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83134/GTX83134_20170912_IHC-P_w_23061322_608.webp "IHC-P analysis of human testis tissue using GTX83134 B-Raf antibody [1H12].")
![WB analysis of HeLa (1), HL60 (2), HepG2 (3) and NIH3T3 (4) cell lysate using GTX83134 B-Raf antibody [1H12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83134/GTX83134_20170912_WB_w_23061322_191.webp "WB analysis of HeLa (1), HL60 (2), HepG2 (3) and NIH3T3 (4) cell lysate using GTX83134 B-Raf antibody [1H12].")
ICC/IF analysis of MCF-7 (left) and HepG2 (right) cells using GTX83134 B-Raf antibody [1H12]. Green : B-Raf Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
B-Raf antibody [1H12]
GTX83134
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetBRAF
Overview
- SupplierGeneTex
- Product NameB-Raf antibody [1H12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1H12
- ConjugateUnconjugated
- Gene ID673
- Target nameBRAF
- Target descriptionB-Raf proto-oncogene, serine/threonine kinase
- Target synonyms94 kDa B-raf protein; B-raf; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; BRAF1; B-RAF1; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1
- HostMouse
- IsotypeIgG1
- Protein IDP15056
- Protein NameSerine/threonine-protein kinase B-raf
- Scientific DescriptionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203