![ICC/IF analysis of COS7 cells transiently transfected with B-Raf plasmid using GTX84812 B-Raf antibody [3A7]. ICC/IF analysis of COS7 cells transiently transfected with B-Raf plasmid using GTX84812 B-Raf antibody [3A7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84812/GTX84812_1240_ICCIF_w_23061420_336.webp)
ICC/IF analysis of COS7 cells transiently transfected with B-Raf plasmid using GTX84812 B-Raf antibody [3A7].
B-Raf antibody [3A7]
GTX84812
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetBRAF
Overview
- SupplierGeneTex
- Product NameB-Raf antibody [3A7] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3A7
- Concentration1.2 mg/ml
- ConjugateUnconjugated
- Gene ID673
- Target nameBRAF
- Target descriptionB-Raf proto-oncogene, serine/threonine kinase
- Target synonymsB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1, serine/threonine-protein kinase B-raf, 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), B-Raf serine/threonine-protein, murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, v-raf murine sarcoma viral oncogene homolog B, v-raf murine sarcoma viral oncogene homolog B1
- HostMouse
- IsotypeIgG2a
- Protein IDP15056
- Protein NameSerine/threonine-protein kinase B-raf
- Scientific DescriptionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84812 B-Raf antibody [3A7].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84812 B-Raf antibody [3A7].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84812/GTX84812_3335_IP_w_23061420_427.webp)
![WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84812 B-Raf antibody [3A7]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84812 B-Raf antibody [3A7]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84812/GTX84812_4641_WB_w_23061420_560.webp)
![WB analysis of various cell lines using GTX84812 B-Raf antibody [3A7]. Loading : 35 ug per lane WB analysis of various cell lines using GTX84812 B-Raf antibody [3A7]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84812/GTX84812_4642_WB_w_23061420_203.webp)


![ICC/IF analysis of MCF-7 (left) and HepG2 (right) cells using GTX83134 B-Raf antibody [1H12]. Green : B-Raf Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX83134/GTX83134_20170912_ICCIF_w_23061322_685.webp)
![ICC/IF analysis of COS7 cells transiently transfected with B-Raf plasmid using GTX84806 B-Raf antibody [1E2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84806/GTX84806_1237_ICCIF_w_23061420_392.webp)
![ICC/IF analysis of COS7 cells transiently transfected with B-Raf plasmid using GTX84807 B-Raf antibody [1D2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84807/GTX84807_1239_ICCIF_w_23061420_886.webp)
![Sandwich ELISA analysis of BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using GTX84820 B-Raf antibody [4A5] (capture antibody) at 5ug/ml and HRP conjugated GTX84814 B-Raf antibody [4B2] (detection antibody) at 0.03ug/ml.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84814/GTX84814_1_ELISA_w_23061420_793.webp)