Sandwich ELISA analysis of BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using GTX84820 B-Raf antibody [4A5] (capture antibody) at 5ug/ml and HRP conjugated GTX84814 B-Raf antibody [4B2] (detection antibody) at 0.03ug/ml.
![IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84814 B-Raf antibody [4B2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84814/GTX84814_3336_IP_w_23061420_716.webp "IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84814 B-Raf antibody [4B2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84814 B-Raf antibody [4B2]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84814/GTX84814_4643_WB_w_23061420_709.webp "WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84814 B-Raf antibody [4B2]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84814 B-Raf antibody [4B2]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84814/GTX84814_4644_WB_w_23061420_451.webp "WB analysis of various cell lines using GTX84814 B-Raf antibody [4B2]. Loading : 35 ug per lane")
Sandwich ELISA analysis of BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using GTX84820 B-Raf antibody [4A5] (capture antibody) at 5ug/ml and HRP conjugated GTX84814 B-Raf antibody [4B2] (detection antibody) at 0.03ug/ml.
B-Raf antibody [4B2]
GTX84814
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetBRAF
Overview
- SupplierGeneTex
- Product NameB-Raf antibody [4B2] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B2
- Concentration1.4 mg/ml
- ConjugateUnconjugated
- Gene ID673
- Target nameBRAF
- Target descriptionB-Raf proto-oncogene, serine/threonine kinase
- Target synonymsB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1, serine/threonine-protein kinase B-raf, 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), B-Raf serine/threonine-protein, murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, v-raf murine sarcoma viral oncogene homolog B, v-raf murine sarcoma viral oncogene homolog B1
- HostMouse
- IsotypeIgG2a
- Protein IDP15056
- Protein NameSerine/threonine-protein kinase B-raf
- Scientific DescriptionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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