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BACE1 antibody [6J26]

GTX52556
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
TargetBACE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    BACE1 antibody [6J26]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6J26
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID23621
  • Target name
    BACE1
  • Target description
    beta-secretase 1
  • Target synonyms
    APP beta-secretase; asp 2; ASP2; aspartyl protease 2; BACE; beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme; HSPC104; memapsin-2; membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP56817
  • Protein Name
    Beta-secretase 1
  • Scientific Description
    This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimers disease patients. [provided by RefSeq, Nov 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BACE1 using anti-BACE1 antibody (A00322-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACE1 antigen affinity purified polyclonal antibody (Catalog # A00322-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACE1 at approximately 70 kDa. The expected band size for BACE1 is at 56 kDa.
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