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BACE1 / BACE Antibody

LS-C812887
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetBACE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BACE1 / BACE Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23621
  • Target name
    BACE1
  • Target description
    beta-secretase 1
  • Target synonyms
    APP beta-secretase; asp 2; ASP2; aspartyl protease 2; BACE; beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme; HSPC104; memapsin-2; membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BACE1 using anti-BACE1 antibody (A00322-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACE1 antigen affinity purified polyclonal antibody (Catalog # A00322-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACE1 at approximately 70 kDa. The expected band size for BACE1 is at 56 kDa.
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