Raw264.7 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Bax antibody [HL236] detects Bax protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44704_20221230_IHC-P_multiple_R_22122821_217.webp "Bax antibody [HL236] detects Bax protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Bax antibody [HL236] detects Bax protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44704_20221230_IHC-P_multiple_M_22122821_567.webp "Bax antibody [HL236] detects Bax protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Wild-type (WT) and Bax knockout (KO) Expi293 cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44048_20230331_WB_KO_watermark_23041023_523.webp "Wild-type (WT) and Bax knockout (KO) Expi293 cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44048_20220128_WB_KO_watermark_w_23061202_522.webp "Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44048_20220318_WB_M_R_w_23061202_358.webp "Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44048_20200821_WB_TPM_watermark_w_23061202_806.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Bax antibody [HL236] detects Bax protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44048_20200918_IHC-P_M_w_23061202_843.webp "Bax antibody [HL236] detects Bax protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635715/GTX635715_44048_20210827_WB_R_w_23061202_735.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Raw264.7 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Bax antibody [HL236]
GTX635715
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetBAX
Overview
- SupplierGeneTex
- Product NameBax antibody [HL236]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL236
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsBCL2L4, apoptosis regulator BAX, BCL2 associated X protein, BCL2-associated X protein omega, Baxdelta2(G8)-RFS protein, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, bcl-2-like protein 4, bcl2-L-4
- HostRabbit
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- Scientific DescriptionThe protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li Y, Dong R, Lu M, et al. Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma. Transl Lung Cancer Res. 2021,10(4):1841-1856. doi: 10.21037/tlcr-21-299Read this paper
- Qi Y, Yang W, Liu S, et al. Cisplatin loaded multiwalled carbon nanotubes reverse drug resistance in NSCLC by inhibiting EMT. Cancer Cell Int. 2021,21(1):74. doi: 10.1186/s12935-021-01771-9Read this paper
- Huang YY, Chen CH, Hsu CH, et al. Inhibiting autophagy potentiates the antitumor efficacy of Euphorbia royleana for canine mammary gland tumors. BMC Vet Res. 2020,16(1):193. doi: 10.1186/s12917-020-02408-1Read this paper
Datasheet
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