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ProductsBack/Bcl x (BCL2L1) Mouse Monoclonal Antibody [Clone ID: OTI4A9]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human BCL2L1 plasmid (RC201314, hBCL2L1), mouse BCL2L1 plasmid (MR202824, mBCL2L1), rat BCL2L1 plasmid (RR200467, rBCL2L1) using anti-BCL2L1 antibody TA500097 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human BCL2L1 plasmid (RC201314, hBCL2L1), mouse BCL2L1 plasmid (MR202824, mBCL2L1), rat BCL2L1 plasmid (RR200467, rBCL2L1) using anti-BCL2L1 antibody TA500097 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human BCL2L1 plasmid (RC201314, hBCL2L1), mouse BCL2L1 plasmid (MR202824, mBCL2L1), rat BCL2L1 plasmid (RR200467, rBCL2L1) using anti-BCL2L1 antibody TA500097 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Bcl x (BCL2L1) Mouse Monoclonal Antibody [Clone ID: OTI4A9]

CF500097
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCL2L1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Bcl x (BCL2L1) Mouse Monoclonal Antibody [Clone ID: OTI4A9]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4A9
  • Gene ID598
  • Target name
    BCL2L1
  • Target description
    BCL2 like 1
  • Target synonyms
    BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52, bcl-2-like protein 1, apoptosis regulator Bcl-X, protein phosphatase 1, regulatory subunit 52
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ07817
  • Protein Name
    Bcl-2-like protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) Bcl-XL mouse monoclonal antibody, clone OTI4A9 (formerly 4A9)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XL antigen affinity purified polyclonal antibody (Catalog # A00181) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-XL at approximately 26-30 kDa. The expected band size for Bcl-XL is at 26 kDa.
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