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ProductsBack/Bcl-6 Polyclonal Antibody
Lane 1:mouse brain lysates probed with Rabbit Anti-Bcl6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1:mouse brain lysates probed with Rabbit Anti-Bcl6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1:mouse brain lysates probed with Rabbit Anti-Bcl6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Bcl-6 Polyclonal Antibody

BS-2734R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBCL6
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Bcl6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID604
  • Target name
    BCL6
  • Target description
    BCL6 transcription repressor
  • Target synonyms
    B cell CLL/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein transcript; BCL5; BCL-5; BCL-6; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; lymphoma-associated zinc finger gene on chromosome 3; protein LAZ-3; ZBTB27; zinc finger and BTB domain-containing protein 27; zinc finger protein 51; zinc finger transcription factor BCL6S; ZNF51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41182
  • Protein Name
    B-cell lymphoma 6 protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Increased levels of CCR7(lo)PD-1(hi) CXCR5+ CD4+ T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure. Gong Q et al., 2017 Dec, Exp Ther Med
    Read more
  • Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis. Szabo K et al., 2017 Sep, Immunol Lett
    Read more

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Figure 1. Western blot analysis of Bcl6 using anti-Bcl6 antibody (A00142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysate, Lane 2: human MCF-7 whole cell lysate, Lane 3: human placenta tissue lysate, Lane 4: human A549 whole cell lysate, Lane 5: human Caco-2 whole cell lysate, Lane 6: human U2OS whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl6 antigen affinity purified polyclonal antibody (Catalog # A00142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl6 at approximately 88KD. The expected band size for Bcl6 is at 79KD.
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