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ProductsBack/beta I Tubulin (5F7) Monoclonal Antibody
Lane 1: K562 cell lysates; Lane 2: Jurkat cell lysates; probed with beta I Tubulin (5F7) Monoclonal Antibody, unconjugated (bsm-33041M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: K562 cell lysates; Lane 2: Jurkat cell lysates; probed with beta I Tubulin (5F7) Monoclonal Antibody, unconjugated (bsm-33041M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: K562 cell lysates; Lane 2: Jurkat cell lysates; probed with beta I Tubulin (5F7) Monoclonal Antibody, unconjugated (bsm-33041M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

beta I Tubulin (5F7) Monoclonal Antibody

BSM-33041M
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTUBB1
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Overview

  • Supplier
    Bioss
  • Product Name
    beta I Tubulin (5F7) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F7
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID81027
  • Target name
    TUBB1
  • Target description
    tubulin beta 1 class VI
  • Target synonyms
    MACTHC1, tubulin beta-1 chain, tubulin, beta 1
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDQ9H4B7
  • Protein Name
    Tubulin beta-1 chain
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tubulin beta using anti-Tubulin beta antibody (A05397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin beta antigen affinity purified polyclonal antibody (Catalog # A05397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin beta at approximately 55 kDa. The expected band size for Tubulin beta is at 50 kDa.
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