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TUBB1 / Tubulin Beta 1 Antibody

LS-C413486
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTUBB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TUBB1 / Tubulin Beta 1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:5000 - 1:10000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81027
  • Target name
    TUBB1
  • Target description
    tubulin beta 1 class VI
  • Target synonyms
    MACTHC1, tubulin beta-1 chain, tubulin, beta 1
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tubulin beta using anti-Tubulin beta antibody (A05397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin beta antigen affinity purified polyclonal antibody (Catalog # A05397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin beta at approximately 55 kDa. The expected band size for Tubulin beta is at 50 kDa.
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