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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta Actin antibody

GTX124214
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse
TargetACTB
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Actin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID60
  • Target name
    ACTB
  • Target description
    actin beta
  • Target synonyms
    BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8, actin, cytoplasmic 1, I(2)-actin, PS1TP5-binding protein 1, beta cytoskeletal actin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60709
  • Protein Name
    Actin, cytoplasmic 1
  • Scientific Description
    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
  • Reactivity
    Drosophila, Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

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  • De Tomi E, Campagnari R, Orlandi E, et al. Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins. Int J Mol Sci. 2022,23(3). doi: 10.3390/ijms23031647
    Read this paper
  • Zanrè V, Campagnari R, Cerulli A, et al. Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation. Int J Mol Sci. 2022,23(3). doi: 10.3390/ijms23031121
    Read this paper
  • Jacobs CL, Badiee RK, Lin MZ. StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins. Nat Methods. 2018,15(7):523-526. doi: 10.1038/s41592-018-0041-z
    Read this paper
  • Peeva V, Blei D, Trombly G, et al. Linear mitochondrial DNA is rapidly degraded by components of the replication machinery. Nat Commun. 2018,9(1):1727. doi: 10.1038/s41467-018-04131-w
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  • Chung HK, Jacobs CL, Huo Y, et al. Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol. 2015,11(9):713-20. doi: 10.1038/nchembio.1869
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  • Butko MT, Savas JN, Friedman B, et al. In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation. Proc Natl Acad Sci U S A. 2013,110(8):E726-35. doi: 10.1073/pnas.1300424110
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