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ProductsBack/beta Catenin antibody [N1N2-2], N-term
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Catenin antibody [N1N2-2], N-term (GTX101435) at 1:300 dilution.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Catenin antibody [N1N2-2], N-term (GTX101435) at 1:300 dilution.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Catenin antibody [N1N2-2], N-term (GTX101435) at 1:300 dilution.

beta Catenin antibody [N1N2-2], N-term

GTX101435
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rabbit, Rat, Zebra Fish
TargetCTNNB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Catenin antibody [N1N2-2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.13 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    CTNNB, EVR7, MRD19, NEDSDV, armadillo, catenin beta-1, catenin (cadherin-associated protein), beta 1, 88kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35222
  • Protein Name
    Catenin beta-1
  • Scientific Description
    Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the contact inhibition signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
  • Reactivity
    Canine, Feline, Human, Mouse, Rabbit, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Şen Ö, Emanet M, Mazzuferi M, et al. Microglia Polarization and Antiglioma Effects Fostered by Dual Cell Membrane-Coated Doxorubicin-Loaded Hexagonal Boron Nitride Nanoflakes. ACS Appl Mater Interfaces. 2023,15(50):58260-58273. doi: 10.1021/acsami.3c17097
    Read this paper
  • Sun L, Zhang M, Jiang J, et al. Neutrophil extracellular traps promote bronchopulmonary dysplasia-like injury in neonatal mice via the WNT/β-catenin pathway. Front Cell Infect Microbiol. 2023,13:1126516. doi: 10.3389/fcimb.2023.1126516
    Read this paper
  • Hsu YC, Chang CC, Hsieh CC, et al. Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease. Int J Mol Sci. 2023,24(8). doi: 10.3390/ijms24087679
    Read this paper
  • Wang X, Ma N, Feng L, et al. Fabrication of bFGF/polydopamine-loaded PEEK implants for improving soft tissue integration by upregulating Wnt/β-catenin signaling. Heliyon. 2023,9(4):e14800. doi: 10.1016/j.heliyon.2023.e14800
    Read this paper
  • Shiba F, Furusho H, Takata T, et al. Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis. Int J Dent. 2022,2022:7398924. doi: 10.1155/2022/7398924
    Read this paper
  • Su MT, Tsai CW, Tsai PY, et al. Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia. Pharmaceuticals (Basel). 2022,15(12). doi: 10.3390/ph15121523
    Read this paper
  • Martin JB, Herman K, Houssin NS, et al. Arvcf Dependent Adherens Junction Stability is Required to Prevent Age-Related Cortical Cataracts. Front Cell Dev Biol. 2022,10:840129. doi: 10.3389/fcell.2022.840129
    Read this paper
  • Li H, Li M, Dong C, et al. Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling. Toxicol Res (Camb). 2022,11(3):437-450. doi: 10.1093/toxres/tfac020
    Read this paper
  • Morita A, Omoya Y, Ito R, et al. Glycyrrhizin and its derivatives promote hepatic differentiation via sweet receptor, Wnt, and Notch signaling. Biochem Biophys Rep. 2021,28:101181. doi: 10.1016/j.bbrep.2021.101181
    Read this paper
  • Park M, Kim HM, Shin HA, et al. Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model. Int J Mol Sci. 2021,22(22). doi: 10.3390/ijms222212529
    Read this paper

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Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
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