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ProductsBack/Goat anti-CTNNB1 / catenin beta-1

Goat anti-CTNNB1 / catenin beta-1

EB12542
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetCTNNB1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CTNNB1 / catenin beta-1 Antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of U251 and A431 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    CTNNB, EVR7, MRD19, NEDSDV, armadillo, catenin beta-1, catenin (cadherin-associated protein), beta 1, 88kDa
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
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Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beta catenin Polyclonal Antibody (bs-1165R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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