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Beta-Tubulin Antibody

MBS856731
MyBioSource
Product group Antibodies
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0.1 ml
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Overview

  • Supplier
    MyBioSource
  • Product Name
    Beta-Tubulin Antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Isotype
    IgG
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (A01857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: chicken heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Tubulin antigen affinity purified polyclonal antibody (Catalog # A01857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 50 kDa. The expected band size for Beta Tubulin is at 50 kDa.
Product group Antibodies
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Biomatik
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RSC96 cells probed with Tubulin-beta Polyclonal Antibody, Unconjugated (bs-0210R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
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ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Porcine, Rat
TargetTUBB
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Product group Antibodies
Cusabio
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ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Chicken, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast
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Immunohistochemical staining of human lymph node shows cytoplasmic positivity in germinal center.
Product group Antibodies
Atlas Antibodies
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ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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Product group Antibodies
LifeSpan BioSciences
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ReactivityHuman, Mouse, Rat
TargetTUBB
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