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ProductsBack/BHMT Mouse Monoclonal Antibody [Clone ID: OTI1E5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BHMT (Cat# RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT(Cat# TA500961). Positive lysates [LY400644] (100ug) and [LC400644] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BHMT (Cat# RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT(Cat# TA500961). Positive lysates [LY400644] (100ug) and [LC400644] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BHMT (Cat# RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT(Cat# TA500961). Positive lysates [LY400644] (100ug) and [LC400644] (20ug) can be purchased separately from OriGene.

BHMT Mouse Monoclonal Antibody [Clone ID: OTI1E5]

CF500961
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBHMT
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    BHMT Mouse Monoclonal Antibody [Clone ID: OTI1E5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E5
  • Gene ID635
  • Target name
    BHMT
  • Target description
    betaine--homocysteine S-methyltransferase
  • Target synonyms
    BHMT1, HEL-S-61p, betaine--homocysteine S-methyltransferase 1, epididymis secretory sperm binding protein Li 61p, phi-crystallin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ93088
  • Protein Name
    Betaine--homocysteine S-methyltransferase 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) BHMT mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of BHMT using anti-BHMT antibody (A06712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BHMT antigen affinity purified polyclonal antibody (Catalog # A06712-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BHMT at approximately 45KD. The expected band size for BHMT is at 45KD.
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