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Formalin-fixed and paraffin embedded rat stomach tissue labeled with Anti-BK channel Polyclonal Antibody, Unconjugated(bs-0729R) 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat stomach tissue labeled with Anti-BK channel Polyclonal Antibody, Unconjugated(bs-0729R) 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat stomach tissue labeled with Anti-BK channel Polyclonal Antibody, Unconjugated(bs-0729R) 1:300 followed by conjugation to the secondary antibody and DAB staining.

KCNMA1/BK channel Polyclonal Antibody

BS-0729R
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetKCNMA1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    BK channel Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3778
  • Target name
    KCNMA1
  • Target description
    potassium calcium-activated channel subfamily M alpha 1
  • Target synonyms
    bA205K10.1; big potassium channel alpha subunit; BK channel alpha subunit; BKCA alpha subunit; BKTM; CADEDS; Calcium-activated potassium channel subunit alpha-1; calcium-activated potassium channel, subfamily M subunit alpha-1; hSlo; IEG16; k(VCA)alpha; KCa1.1; LIWAS; MaxiK; maxi-K channel HSLO; mSLO1; PNKD3; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; SAKCA; SLO; slo homolog; SLO1; SLO-ALPHA; slowpoke homolog; stretch-activated Kca channel
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12791
  • Protein Name
    Calcium-activated potassium channel subunit alpha-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Time-course of glial changes in the hyperhomocysteinemia model of vascular cognitive impairment and dementia (VCID). Sudduth TL et al., 2017 Jan 26, Neuroscience
    Read more

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Figure 1. Western blot analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U87 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNMA1 antigen affinity purified polyclonal antibody (Catalog # PB9227) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNMA1 at approximately 110-138 kDa. The expected band size for KCNMA1 is at 138KD.
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