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Western Blot analysis of HeLa cells using MaxiKalpha Polyclonal Antibody
Western Blot analysis of HeLa cells using MaxiKalpha Polyclonal Antibody
Western Blot analysis of HeLa cells using MaxiKalpha Polyclonal Antibody

KCNMA1 Antibody

CSB-PA003200
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNMA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNMA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3778
  • Target name
    KCNMA1
  • Target description
    potassium calcium-activated channel subfamily M alpha 1
  • Target synonyms
    bA205K10.1; big potassium channel alpha subunit; BK channel alpha subunit; BKCA alpha subunit; BKTM; CADEDS; Calcium-activated potassium channel subunit alpha-1; calcium-activated potassium channel, subfamily M subunit alpha-1; hSlo; IEG16; k(VCA)alpha; KCa1.1; LIWAS; MaxiK; maxi-K channel HSLO; mSLO1; PNKD3; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; SAKCA; SLO; slo homolog; SLO1; SLO-ALPHA; slowpoke homolog; stretch-activated Kca channel
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12791
  • Protein Name
    Calcium-activated potassium channel subunit alpha-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U87 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNMA1 antigen affinity purified polyclonal antibody (Catalog # PB9227) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNMA1 at approximately 110-138 kDa. The expected band size for KCNMA1 is at 138KD.
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