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ProductsBack/KCNMA1 / BK Antibody

KCNMA1 / BK Antibody

LS-C750254
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetKCNMA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KCNMA1 / BK Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 17kDa/130kDa/134kDa/135kDa/137kDa/138kDa, while the observed MW by Western blot was 137kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 17kDa/130kDa/134kDa/135kDa/137kDa/138kDa, while the observed MW by Western blot was 137kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3778
  • Target name
    KCNMA1
  • Target description
    potassium calcium-activated channel subfamily M alpha 1
  • Target synonyms
    BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1, Calcium-activated potassium channel subunit alpha-1, calcium-activated potassium channel subunit alpha-1, BK channel alpha subunit, BKCA alpha subunit, big potassium channel alpha subunit, calcium-activated potassium channel, subfamily M subunit alpha-1, k(VCA)alpha, maxi-K channel HSLO, potassium channel, calcium activated large conductance subfamily M alpha, member 1, potassium large conductance calcium-activated channel, subfamily M, alpha member 1, slo homolog, slowpoke homolog, stretch-activated Kca channel
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U87 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNMA1 antigen affinity purified polyclonal antibody (Catalog # PB9227) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNMA1 at approximately 110-138 kDa. The expected band size for KCNMA1 is at 138KD.
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