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ProductsBack/BLNK Mouse Monoclonal Antibody [Clone ID: 5G9]
Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)

BLNK Mouse Monoclonal Antibody [Clone ID: 5G9]

AM06644SU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetBLNK
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    BLNK Mouse Monoclonal Antibody [Clone ID: 5G9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5G9
  • Gene ID29760
  • Target name
    BLNK
  • Target description
    B cell linker
  • Target synonyms
    AGM4, BASH, BLNK-S, LY57, SLP-65, SLP65, bca, B-cell linker protein, B cell adaptor containing SH2 domain, B-cell activation, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, Src homology [SH2] domain-containing leukocyte protein of 65 kD, cytoplasmic adapter protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8WV28
  • Protein Name
    B-cell linker protein
  • Scientific Description
    BLNK mouse monoclonal antibody, clone 5G9, Ascites
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of BLNK using anti-BLNK antibody (A03630-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLNK antigen affinity purified polyclonal antibody (Catalog # A03630-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLNK at approximately 70 kDa. The expected band size for BLNK is at 70 kDa.
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