More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/BMAL1 antibody [N1N3]
Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BMAL1 antibody [N1N3]

GTX105060
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetBMAL1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    BMAL1 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.65 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID406
  • Target name
    BMAL1
  • Target description
    basic helix-loop-helix ARNT like 1
  • Target synonyms
    ARNTL, ARNTL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5, basic helix-loop-helix ARNT-like protein 1, ARNT-like protein 1, brain and muscle, PAS domain containing 3, PAS domain-containing protein 3, aryl hydrocarbon receptor nuclear translocator like, aryl hydrocarbon receptor nuclear translocator-like protein 1, bHLH-PAS protein JAP3, basic helix-loop-helix family member e5, basic-helix-loop-helix-PAS orphan MOP3, basic-helix-loop-helix-PAS protein MOP3, brain and muscle ARNT-like 1, class E basic helix-loop-helix protein 5, member of PAS protein 3, member of PAS superfamily 3, mutant basic helix-loop-helix ARNT-like protein 1, testis tissue sperm-binding protein Li 50e
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00327
  • Protein Name
    Basic helix-loop-helix ARNT-like protein 1
  • Scientific Description
    The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-BMAL1/ARNTL Antibody Picoband(r)A00260-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetBMAL1
  • SizePrice
WB analysis of BMAL1(AA: 1-310)-hIgGFc transfected HEK293 cell lysate using GTX83281 BMAL1 antibody [1C5].
Product group Antibodies
GeneTex
BMAL1 antibody [1C5]GTX83281
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetBMAL1
  • SizePrice
FACS analysis of Jurkat cells using GTX84851 BMAL1 antibody [3G9]. Red : Primary antibody Blue : Negative control antibody
Product group Antibodies
GeneTex
BMAL1 antibody [3G9]GTX84851
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetBMAL1
  • SizePrice
IHC-P analysis of lung tissue using GTX84852 BMAL1 antibody [1C11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
Product group Antibodies
GeneTex
BMAL1 antibody [1C11]GTX84852
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetBMAL1
  • SizePrice
ICC/IF analysis of COS7 cells transiently transfected with BMAL1 plasmid using GTX84853 BMAL1 antibody [1D1].
Product group Antibodies
GeneTex
BMAL1 antibody [1D1]GTX84853
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetBMAL1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMAL1 Polyclonal Antibody, Unconjugated (bs-3750R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
BMAL1 Polyclonal Antibodybs-3750R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetBMAL1
  • SizePrice
Immunofluorescence staining of PC-3 cells with CSB-PA002123LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
ARNTL AntibodyCSB-PA002123LA01HU
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetBMAL1
  • SizePrice
Product group Antibodies
Assay Genie
BMAL1 Monoclonal AntibodyCAB4714
ReactivityRat
TargetBMAL1
  • SizePrice
WB analysis of human brain (cerebellum) lysate using GTX88473 BMAL1 antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
Product group Antibodies
GeneTex
BMAL1 antibody, InternalGTX88473
ApplicationsWestern Blot
ReactivityHuman
TargetBMAL1
  • SizePrice
Anti-BMAL1 Antibody
Product group Antibodies
Antibodies.com
Anti-BMAL1 AntibodyA97688
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice