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Anti-BMAL1 Antibody

Anti-BMAL1 Antibody

A97688
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-BMAL1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to BMAL1.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-BMAL1/ARNTL Antibody Picoband(r)A00260-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetBMAL1
  • SizePrice
Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
BMAL1 antibody [N1N3]GTX105060
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetBMAL1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMAL1 Polyclonal Antibody, Unconjugated (bs-3750R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
BMAL1 Polyclonal Antibodybs-3750R
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ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetBMAL1
  • SizePrice
Immunofluorescence staining of PC-3 cells with CSB-PA002123LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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ARNTL AntibodyCSB-PA002123LA01HU
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TargetBMAL1
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Product group Antibodies
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BMAL1 Monoclonal AntibodyCAB4714
ReactivityRat
TargetBMAL1
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Product group Antibodies
MedChem Express
BMAL1 Antibody (YA581, Rabbit)HY-P80033
TargetBMAL1
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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
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TargetBMAL1
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Goat anti-BMAL1 / ARNTL AntibodyEB09178
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ARNTL / BMAL1 AntibodyLS-C401453
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TargetBMAL1
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