
WB analysis of various sample lysates using GTX32472 Bmi1 antibody. Dilution : 1:5000 Loading : 25μg per lane
Bmi1 antibody
GTX32472
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBMI1
Overview
- SupplierGeneTex
- Product NameBmi1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. IP: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID648
- Target nameBMI1
- Target descriptionBMI1 proto-oncogene, polycomb ring finger
- Target synonymsFLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1, polycomb complex protein BMI-1, B lymphoma Mo-MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, BMI1 polycomb ring finger proto-oncogene, murine leukemia viral (bmi-1) oncogene homolog, polycomb group RING finger protein 4, polycomb group protein Bmi1, ring finger protein 51
- HostRabbit
- IsotypeIgG
- Protein IDP35226
- Protein NamePolycomb complex protein BMI-1
- Scientific DescriptionThis gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203





![Western blot using GeneTex's Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 μg of a U2OS whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4o C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX45791/GTX45791_20160330_WB_w_23060820_955.webp)


