
WB analysis of human skeletal muscle tissue lysate using GTX48576 Bmi1 antibody.
Bmi1 antibody
GTX48576
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Chicken, Feline, Human, Mouse, Primate, Rabbit, Rat, Zebra Fish
TargetBMI1
Overview
- SupplierGeneTex
- Product NameBmi1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 2 microg/ml. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID648
- Target nameBMI1
- Target descriptionBMI1 proto-oncogene, polycomb ring finger
- Target synonymsFLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1, polycomb complex protein BMI-1, B lymphoma Mo-MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, BMI1 polycomb ring finger proto-oncogene, murine leukemia viral (bmi-1) oncogene homolog, polycomb group RING finger protein 4, polycomb group protein Bmi1, ring finger protein 51
- HostRabbit
- IsotypeIgG
- Protein IDP35226
- Protein NamePolycomb complex protein BMI-1
- Scientific DescriptionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
- ReactivityBovine, Chicken, Feline, Human, Mouse, Primate, Rabbit, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203





![Western blot using GeneTex's Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 μg of a U2OS whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4o C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX45791/GTX45791_20160330_WB_w_23060820_955.webp)

