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Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody (GTX28399) at 1μg/ml.
Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody (GTX28399) at 1μg/ml.
Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody (GTX28399) at 1μg/ml.

BNIP3L antibody

GTX28399
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBNIP3L
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Overview

  • Supplier
    GeneTex
  • Product Name
    BNIP3L antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID665
  • Target name
    BNIP3L
  • Target description
    BCL2 interacting protein 3 like
  • Target synonyms
    BNIP3a, NIP3L, NIX, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BCL2/adenovirus E1B 19-kd protein-interacting protein 3a, BCL2/adenovirus E1B 19kDa interacting protein 3 like, NIP-3-like protein X, NIP3-like protein X, adenovirus E1B19k-binding protein B5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60238
  • Protein Name
    BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
  • Scientific Description
    This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c. [provided by RefSeq, Feb 2015]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BNIP3L using anti-BNIP3L antibody (A03107-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3L antigen affinity purified polyclonal antibody (Catalog # A03107-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BNIP3L at approximately 35 kDa. The expected band size for BNIP3L is at 24 kDa.
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BNIP3L antibody detects BNIP3L protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. BNIP3L stained by BNIP3L antibody (GTX111876) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TargetBNIP3L
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BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BNIP3L stained by BNIP3L antibody [HL1203] (GTX636515) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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