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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA072508(BPIFB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA072508(BPIFB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA072508(BPIFB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

BPIFB1 Antibody

CSB-PA072508
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBPIFB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    BPIFB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92747
  • Target name
    BPIFB1
  • Target description
    BPI fold containing family B member 1
  • Target synonyms
    BPI fold-containing family B member 1; C20orf114; long palate, lung and nasal epithelium carcinoma associated 1; long palate, lung and nasal epithelium carcinoma-associated protein 1; LPLUNC1; VEMSGP; von Ebner minor salivary gland protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDL5
  • Protein Name
    BPI fold-containing family B member 1
  • Scientific Description
    The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Jurkat cell lysates probed with LPLUNC1 Polyclonal Antibody, Unconjugated (bs-0976R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of BPIFB1 using anti-BPIFB1 antibody (A08872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BPIFB1 antigen affinity purified polyclonal antibody (Catalog # A08872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BPIFB1 at approximately 52 kDa. The expected band size for BPIFB1 is at 52 kDa.
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Immunohistochemical staining of human small intestine shows strong positivity in paneth cells.
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ELISA analysis of antigen using GTX60533 LPlunc1 antibody [2A5]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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