More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/BTLA / CD272 Antibody (clone PK18.6, Azide-free, Low Endotoxin)

BTLA / CD272 Antibody (clone PK18.6, Azide-free, Low Endotoxin)

LS-C812181
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetBTLA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BTLA / CD272 Antibody (clone PK18.6, Azide-free, Low Endotoxin)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 microg per 106 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PK18.6
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID151888
  • Target name
    BTLA
  • Target description
    B and T lymphocyte associated
  • Target synonyms
    B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA1; CD272
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-BTLA Antibody144-08377
ApplicationsWestern Blot
TargetBTLA
  • SizePrice
Product group Antibodies
Assay Genie
BTLA AntibodyCAB13449
TargetBTLA
  • SizePrice
Product group Antibodies
Biomatik
B And T-Lymphocyte Attenuator (BTLA) Polyclonal AntibodyCAU25438
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetBTLA
  • SizePrice
Western Blot analysis of K562 cells using CD272 Polyclonal Antibody
Product group Antibodies
Cusabio
BTLA AntibodyCSB-PA007471
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetBTLA
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded mouse lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tCD272 Polyclonal Antibody, Unconjugated (bs-0624R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
Bioss Antibodies
CD272 Polyclonal AntibodyBS-0624R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetBTLA
  • SizePrice
Anti-BTLA Antibody
Product group Antibodies
Antibodies.com
Anti-BTLA AntibodyA43801
ApplicationsWestern Blot
  • SizePrice
Immunohistochemistry analysis in human tonsil and pancreas tissues using HPA062029 antibody. Corresponding BTLA RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-BTLA AntibodyHPA062029
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetBTLA
  • SizePrice
Figure 1. Western blot analysis of CD272 using anti-CD272 antibody (A03149). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysate, Lane 2: human Jurkat whole cell lysate, Lane 3: human CCRF-CEM whole cell lysate, Lane 4: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD272 antigen affinity purified polyclonal antibody (Catalog # A03149) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD272 at approximately 65KD. The expected band size for CD272 is at 33KD.
Product group Antibodies
Boster Bio
Anti-CD272/BTLA Antibody Picoband(r)A03149-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetBTLA
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-BTLA (human), mAb (6F4)AG-20B-0049
ApplicationsFlow Cytometry, ELISA
TargetBTLA
  • SizePrice
Product group Antibodies
GeneTex
CD272 antibodyGTX112783
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetBTLA
  • SizePrice