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ProductsBack/BTLA / CD272 Antibody (clone PK18.6)

BTLA / CD272 Antibody (clone PK18.6)

LS-C770041
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetBTLA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BTLA / CD272 Antibody (clone PK18.6)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 microg per 10E6 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 µg per 10E6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PK18.6
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID151888
  • Target name
    BTLA
  • Target description
    B and T lymphocyte associated
  • Target synonyms
    B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA1; CD272
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD272 using anti-CD272 antibody (A03149). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysate, Lane 2: human Jurkat whole cell lysate, Lane 3: human CCRF-CEM whole cell lysate, Lane 4: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD272 antigen affinity purified polyclonal antibody (Catalog # A03149) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD272 at approximately 65KD. The expected band size for CD272 is at 33KD.
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