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ProductsBack/BUB1B antibody [11D7]
ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84767 BUB1B antibody [11D7].
ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84767 BUB1B antibody [11D7].
ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84767 BUB1B antibody [11D7].

BUB1B antibody [11D7]

GTX84767
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetBUB1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    BUB1B antibody [11D7] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11D7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID701
  • Target name
    BUB1B
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Target synonyms
    Bub1A; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 homolog beta; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; SSK1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO60566
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
  • Scientific Description
    Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BubR1/BUB1B antigen affinity purified polyclonal antibody (Catalog # A01564-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 120-130KD. The expected band size for BubR1/BUB1B is at 120-130KD.
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