ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84768 BUB1B antibody [11G2].
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX84768 BUB1B antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84768/GTX84768_3091_IHC-P_w_23061420_788.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX84768 BUB1B antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of thyroid carcinoma tissue using GTX84768 BUB1B antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84768/GTX84768_3092_IHC-P_w_23061420_802.webp "IHC-P analysis of thyroid carcinoma tissue using GTX84768 BUB1B antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![WB analysis of HEK293T cells transfected with BUB1B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84768 BUB1B antibody [11G2]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84768/GTX84768_4615_WB_w_23061420_603.webp "WB analysis of HEK293T cells transfected with BUB1B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84768 BUB1B antibody [11G2]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84768 BUB1B antibody [11G2]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84768/GTX84768_4616_WB_w_23061420_725.webp "WB analysis of various cell lines using GTX84768 BUB1B antibody [11G2]. Loading : 35 ug per lane")
ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84768 BUB1B antibody [11G2].
BUB1B antibody [11G2]
GTX84768
Overview
- SupplierGeneTex
- Product NameBUB1B antibody [11G2] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- Clone ID11G2
- Concentration1 mg/ml
- FormulationLiquid
- Gene ID701
- Target nameBUB1B
- Target descriptionBUB1 mitotic checkpoint serine/threonine kinase B
- Target synonymsBub1A; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 homolog beta; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; SSK1
- Protein IDO60566
- Protein NameMitotic checkpoint serine/threonine-protein kinase BUB1 beta
- Scientific DescriptionEssential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203