More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI6E5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B. Positive lysates LY420068 (100ug) and LC420068 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B. Positive lysates LY420068 (100ug) and LC420068 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B. Positive lysates LY420068 (100ug) and LC420068 (20ug) can be purchased separately from OriGene.

BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI6E5]

CF500607
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBUB1B
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI6E5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI6E5
  • Gene ID701
  • Target name
    BUB1B
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Target synonyms
    BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1, mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUB1B, mitotic checkpoint serine/threonine kinase, MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta, mitotic checkpoint kinase MAD3L
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO60566
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
  • Scientific Description
    Carrier-free (BSA/glycerol-free) BUB1B mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
SMAP1 AntibodyCAB17750
ReactivityHuman
TargetBUB1B
  • SizePrice
Western Blot analysis of HeLa cells using BubR1 Polyclonal Antibody
Product group Antibodies
Cusabio
BUB1B AntibodyCSB-PA001071
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetBUB1B
  • SizePrice
Figure 1. Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BubR1/BUB1B antigen affinity purified polyclonal antibody (Catalog # A01564-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 120-130KD. The expected band size for BubR1/BUB1B is at 120-130KD.
Product group Antibodies
Boster Bio
Anti-BubR1/BUB1B Antibody Picoband(r)A01564-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBUB1B
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Mad3 [N129A/6R]Ab02313-10.0
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetBUB1B
  • SizePrice
Anti-BUB1B Antibody
Product group Antibodies
Antibodies.com
Anti-BUB1B AntibodyA99162
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
BUB1B / BubR1 AntibodyLS-C760826
ApplicationsWestern Blot
ReactivityHuman
TargetBUB1B
  • SizePrice
Product group Antibodies
MedChem Express
BUBR1 Antibody (YA2757, Rabbit)HY-P83012
TargetBUB1B
  • SizePrice
Product group Antibodies
Biomatik
BUB1B Polyclonal AntibodyCAC14527
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetBUB1B
  • SizePrice
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal (GTX111289) diluted at 1:500.
Product group Antibodies
GeneTex
BUB1B antibody [N2C1], InternalGTX111289
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetBUB1B
  • SizePrice
Product group Antibodies
Bioss
BubR1 Recombinant Antibody, AbBy Fluor-488 ConjugatedBSM-61498R-BF488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetBUB1B
  • SizePrice