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ProductsBack/c-Jun (JUN) Mouse Monoclonal Antibody [Clone ID: OTI1C8]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C-Jun (RC209804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C-Jun. Positive lysates LY400825 (100ug) and LC400825 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C-Jun (RC209804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C-Jun. Positive lysates LY400825 (100ug) and LC400825 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C-Jun (RC209804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C-Jun. Positive lysates LY400825 (100ug) and LC400825 (20ug) can be purchased separately from OriGene.

c-Jun (JUN) Mouse Monoclonal Antibody [Clone ID: OTI1C8]

CF500226
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetJUN
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    c-Jun (JUN) Mouse Monoclonal Antibody [Clone ID: OTI1C8]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1C8
  • Gene ID3725
  • Target name
    JUN
  • Target description
    Jun proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, AP1, c-Jun, cJUN, p39, transcription factor Jun, Jun activation domain binding protein, activator protein 1, enhancer-binding protein AP1, jun oncogene, proto-oncogene c-Jun, proto-oncogene cJun, transcription factor AP-1, transcription factor AP-1 subunit Jun, v-jun avian sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP05412
  • Protein Name
    Transcription factor Jun
  • Scientific Description
    Carrier-free (BSA/glycerol-free) C-Jun mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (Catalog # A02038-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Jun/JUN at approximately 43 kDa. The expected band size for c-Jun/JUN is at 43 kDa.
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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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