c-Jun (phospho Ser73) antibody [HL2713] detects c-Jun (phospho Ser73) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639474/GTX639474_45313_20240216_WB_treatment_anisomycin_24022619_627.webp "Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Specificity analysis was performed by coating the plate with recombinant c-Jun non-phospho peptide and c-Jun (phospho Ser73) peptide (250 ng/mL). Coated protein was probed with c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639474/GTX639474_45313_20250523_ELISA_Indirect_Multiple_Bar_Specificity_25052800_850.webp "Specificity analysis was performed by coating the plate with recombinant c-Jun non-phospho peptide and c-Jun (phospho Ser73) peptide (250 ng/mL). Coated protein was probed with c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
c-Jun (phospho Ser73) antibody [HL2713] detects c-Jun (phospho Ser73) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
c-Jun (phospho Ser73) antibody [HL2713]
GTX639474
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetJUN
Overview
- SupplierGeneTex
- Product Namec-Jun (phospho Ser73) antibody [HL2713]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2713
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3725
- Target nameJUN
- Target descriptionJun proto-oncogene, AP-1 transcription factor subunit
- Target synonymsAP-1, AP1, c-Jun, cJUN, p39, transcription factor Jun, Jun activation domain binding protein, activator protein 1, enhancer-binding protein AP1, jun oncogene, proto-oncogene c-Jun, proto-oncogene cJun, transcription factor AP-1, transcription factor AP-1 subunit Jun, v-jun avian sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog
- HostRabbit
- IsotypeIgG
- Protein IDP05412
- Protein NameTranscription factor Jun
- Scientific DescriptionThis gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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References
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