Indirect ELISA analysis was performed by coating the plate with recombinant Human C Reactive Protein, His tag (GTX138453-pro) (144.14-2.25 nM). Coated protein was probed with C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
![Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101262/GTX101262_45252_20231208_WB_R_plasma_23121122_431.webp "Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101262/GTX101262_45252_20231201_WB_M_plasma_23121122_354.webp "Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101262/GTX101262_45252_20231201_WB_plasma_23121122_917.webp "Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![C Reactive Protein antibody [N1C3] detects secreted C Reactive Protein protein by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. C Reactive Protein stained by C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101262/GTX101262_45119_20231201_IHC-P_24010223_883.webp "C Reactive Protein antibody [N1C3] detects secreted C Reactive Protein protein by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. C Reactive Protein stained by C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Sandwich ELISA detection of recombinant Human C Reactive Protein, His tag (GTX138453-pro) using antibodies as below. Capture: C Reactive Protein antibody [GT55] (GTX641407) (5 μg/mL) Detection: C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX101262/GTX101262_45259_20241220_ELISA_PAIR_2_24123021_561.webp "Sandwich ELISA detection of recombinant Human C Reactive Protein, His tag (GTX138453-pro) using antibodies as below. Capture: C Reactive Protein antibody [GT55] (GTX641407) (5 μg/mL) Detection: C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL)")
![Sandwich ELISA detection of recombinant Human C Reactive Protein, His tag (GTX138453-pro) using antibodies as below. Capture: C Reactive Protein antibody [GT54] (GTX641406) (5 μg/mL) Detection: C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX101262/GTX101262_45259_20241220_ELISA_PAIR_24123021_810.webp "Sandwich ELISA detection of recombinant Human C Reactive Protein, His tag (GTX138453-pro) using antibodies as below. Capture: C Reactive Protein antibody [GT54] (GTX641406) (5 μg/mL) Detection: C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL)")
Indirect ELISA analysis was performed by coating the plate with recombinant Human C Reactive Protein, His tag (GTX138453-pro) (144.14-2.25 nM). Coated protein was probed with C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
C Reactive Protein antibody [N1C3]
GTX101262
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCRP
Overview
- SupplierGeneTex
- Product NameC Reactive Protein antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.24 mg/ml
- ConjugateUnconjugated
- Gene ID1401
- Target nameCRP
- Target descriptionC-reactive protein
- Target synonymsPTX1, C-reactive protein, C-reactive protein, pentraxin-related, pentraxin 1
- HostRabbit
- IsotypeIgG
- Protein IDP02741
- Protein NameC-reactive protein
- Scientific DescriptionThe protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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