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C1R Antibody

CAB15650
Assay Genie
TargetBMP4
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    C1R Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID652
  • Target name
    BMP4
  • Target description
    bone morphogenetic protein 4
  • Target synonyms
    BMP2B; BMP2B1; bone morphogenetic protein 2B; bone morphogenetic protein 4; MCOPS6; OFC11; ZYME
  • Host
    Rabbit
  • Protein IDP12644
  • Protein Name
    Bone morphogenetic protein 4
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMP4 using anti-BMP4 antibody (A00321). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human BMP4 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody (Catalog # A00321) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP4 at approximately 22KD. The expected band size for BMP4 is at 13KD.
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