Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C5b-9 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C5b-9 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
C5b-9 Polyclonal Antibody
BS-2673R
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetC9
Overview
- SupplierBioss Antibodies
- Product NameC5b-9 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierIHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID735
- Target nameC9
- Target descriptioncomplement C9
- Target synonymsARMD15; C9D; complement component 9; complement component C9
- HostRabbit
- IsotypeIgG
- Protein IDP02748
- Protein NameComplement component C9
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Widespread cortical demyelination of both hemispheres can be induced by injection of pro-inflammatory cytokines via an implanted catheter in the cortex of MOG-immunized rats. Uecal M et al., 2017 Aug, Exp NeurolRead more
- Age-related increases in amyloid beta and membrane attack complex: evidence of inflammasome activation in the rodent eye. Zhao T et al., 2015 Jun 24, J NeuroinflammationRead more