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Western Blot analysis of COLO205 cells using C9 Polyclonal Antibody
Western Blot analysis of COLO205 cells using C9 Polyclonal Antibody
Western Blot analysis of COLO205 cells using C9 Polyclonal Antibody

C9 Antibody

CSB-PA001095
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetC9
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Overview

  • Supplier
    Cusabio
  • Product Name
    C9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID735
  • Target name
    C9
  • Target description
    complement C9
  • Target synonyms
    ARMD15, C9D, complement component C9, complement component 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02748
  • Protein Name
    Complement component C9
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Complement C9 using anti-Complement C9 antibody (A01010-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Complement C9 antigen affinity purified polyclonal antibody (Catalog # A01010-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Complement C9 at approximately 80KD. The expected band size for Complement C9 is at 63KD.
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