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Western blot analysis of DFF40 in Jurkat cell lysate with CAD antibody (GTX28407) at (A) 0.5, (B) 1 and (C) 2 g/ml.
Western blot analysis of DFF40 in Jurkat cell lysate with CAD antibody (GTX28407) at (A) 0.5, (B) 1 and (C) 2 g/ml.
Western blot analysis of DFF40 in Jurkat cell lysate with CAD antibody (GTX28407) at (A) 0.5, (B) 1 and (C) 2 g/ml.

CAD antibody

GTX28407
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDffb
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Overview

  • Supplier
    GeneTex
  • Product Name
    CAD antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2microg/ml. ICC/IF: 10microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID13368
  • Target name
    Dffb
  • Target description
    DNA fragmentation factor, beta subunit
  • Target synonyms
    40kDa, 5730477D02Rik, CAD, CPAN, DFF40, Didff, DNA fragmentation factor subunit beta, DFF-40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor, 40 kD, beta subunit, DNase inhibited by DNA fragmentation factor, caspase-activated DNase, caspase-activated deoxyribonuclease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO54788
  • Protein Name
    DNA fragmentation factor subunit beta
  • Scientific Description
    Enables DNA binding activity and nuclease activity. Predicted to be involved in apoptotic DNA fragmentation; apoptotic chromosome condensation; and negative regulation of apoptotic DNA fragmentation. Predicted to be located in chromatin; cytosol; and nuclear lumen. Predicted to be part of protein-containing complex. Orthologous to human DFFB (DNA fragmentation factor subunit beta). [provided by Alliance of Genome Resources, Jul 2025]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of CAD using anti-CAD antibody (A00463-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 270KD. The expected band size for CAD is at 243KD.
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