Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230512_WB_M_tissue_23051702_945.webp "Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20230714_WB_TPM_watermark_23071822_955.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230707_IHC-P_R_23071822_814.webp "Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230707_IHC-P_23071822_932.webp "Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20230908_WB_C_23091319_428.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000 and competitor's antibody (Clone 5C10, ADD) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20240301_WB_competitor_watermark_24030600_300.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000 and competitor's antibody (Clone 5C10, ADD) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Immunoprecipitation of Calcium Sensing Receptor protein from TT whole cell extract using 5 μg of Calcium Sensing Receptor antibody [HL2357] (GTX638563). Western blot analysis was performed using Calcium Sensing Receptor antibody [HL2357] (GTX638563). EasyBlot HRP-conjugated anti FALSE.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20240503_IP_24050701_823.webp "Immunoprecipitation of Calcium Sensing Receptor protein from TT whole cell extract using 5 μg of Calcium Sensing Receptor antibody [HL2357] (GTX638563). Western blot analysis was performed using Calcium Sensing Receptor antibody [HL2357] (GTX638563). EasyBlot HRP-conjugated anti FALSE.")
![Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20251114_IHC-P_M_25112800_349.webp "Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20251114_IHC-P_M_Multiple_RPKM_25112800_669.webp "Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Calcium Sensing Receptor antibody [HL2357]
GTX638563
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFeline, Human, Mouse, Rat
TargetCASR
Overview
- SupplierGeneTex
- Product NameCalcium Sensing Receptor antibody [HL2357]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2357
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID846
- Target nameCASR
- Target descriptioncalcium sensing receptor
- Target synonymsCAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1, hCasR, extracellular calcium-sensing receptor, parathyroid Ca(2+)-sensing receptor 1, parathyroid cell calcium-sensing receptor 1
- HostRabbit
- IsotypeIgG
- Protein IDP41180
- Protein NameExtracellular calcium-sensing receptor
- Scientific DescriptionThe protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]
- ReactivityFeline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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